11. Smart production of spirulina (Spirulina platensis) using supernatant of digested rotten potato (Solanum tuberosum)

Abstract

The study was conducted to evaluate the culture and growth performance of spirulina (Spirulina platensis) in supernatant of three different amounts of digested rotten potato (DRP), and Kosaric medium (KM) as control in 16 days after 26 days digestion. Three different concentrations such as 20, 40 and 60% of DRP were used. Spirulina was inoculated in supernatant DRP along with 9.0 g/L NaHCO3 and micronutrients, and KM for a period of 14 days. The cell weight of spirulina was attained a maximum of 12.42 ± 0.21 mg/L in KM followed by 8.352 ± 0.21, 6.256 ± 2.34 and 9.505 ± 0.43 mg/L in supernatant of 40, 20 and 60% DRP, respectively on the 10th day of culture. Similar trend was also observed in the cases of optical density, chlorophyll a, total biomass, specific growth rates. Cell weight of spirulina grown in the media were highly significant (p<0.01) and correlated with the chlorophyll a content and total biomass. The growth performance of spirulina grown in supernatant of 60% DRP was significantly higher than that of spirulina grown in supernatant of 20
and 40% DRP. Therefore, mass culture of spirulina may be done in supernatant of 60% DRP.